• University of New South Wales

• BABS 3031

 PART A [BIOPROCESS KINETICS USING BERKLEY MADONNA] SIMULATION 1- BATCH FERMENTATION. 1. Start the simulation  When does S reach zero? - The time at which the substrate level reaches zero is at 23.24 hr.  What is the biomass level at this time when S reaches 0? - The level of biomass when the substrate level reaches zero is 8.01 kg/m3  Why does the biomass stop increasing? - As it is a substrate limited process , Therefore according to the equation - μ= μmax∗S KS+S , when substrate reaches out μ Equals to zero. And as RX = X* μ , when μ equals to zero , biomass stops increasing.  Describe what you see with product formation - Unlike biomass, the product formation keeps increasing even after the substrate level reaches zero. Thus stating that it is a non growth associated product formation. - - 2- Effect of changing S0  Effect on final cell yield - As the initial concentration of substrate increases , the final cell yield also increases.  Effect on final product yield - With the increase in initial concentration of substrate , the amount of product yield increases.  Time taken for cell growth to stop - With the increase in S0 , the time required for the cell growth to stop increases.  Do a screen capture of the profiles and save them in your report