Biology 204Spring 2019/REAProblem Set 3: Enzyme kinetics and mechanismQuestions:Question 5 provides you with an introduction to the pros and cons of the linear transformations that are mostcommonly employed for estimating Km and Vmax (if not in the real world, in textbooks and MCATs, GREs andthe like). In working your way through this question, you will discover some of the merits and inadequacies
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Biology 204
Spring 2019/REA
Problem Set 3: Enzyme kinetics and mechanism
Questions:
Question 5 provides you with an introduction to the pros and cons of the linear transformations that are most
commonly employed for estimating Km and Vmax (if not in the real world, in textbooks and MCATs, GREs and
the like). In working your way through this question, you will discover some of the merits and inadequacies of
these transformations for yourself. Questions 6, 7, 8, 11 and 12 have their origins in research that was actually
conducted except that a couple of the scenarios played out with a slightly different outcome. Question 7
provides you with a concrete, real-world example of how Michaelis-Menten kinetics might be applied to
bisubstrate reactions. Questions 8 and 9 illustrate the kinetic basis of the efficacy of ACE inhibitors for the
treatment of hypertension, and lithium for the management of bipolar disorder (with some surprises!); two drugs
that rank among the most widely prescribed in the United States and Europe. Question 12 provides you with an
example of a widely applied approach for measuring the activity of enzymes spectrophotometrically even when
the reactions catalyzed are not chromogenic.
1. One µg of a pure enzyme (MW = 92,000) catalyzed a reaction at a rate of 0.5 µmole/min
under optimal conditions. Calculate (a) the specific activity of the enzyme in terms of
units/mg protein and units/mol and (b) the turnover number. (c) How long does each
catalytic cycle take? One International Unit (IU) of enzyme activity is the amount that
catalyzes the formation of 1 µmol of product per minute under defined conditions.
This question is intended to give you a sense of scaling. Refer to the course text if you need to refresh
your memory of the basics of enzyme kinetics, specifically the concepts of turnover number and turnover
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