BIOL1133 Biological Sciences Laboratory CoursePractical 1DNA sequencing and analysisDate of practical: 24-1-2013Plasmid DNA no.: 16 Abstract Automated sequencing with dye-terminator was used in this practical. Different length of DNA fragments was synthesized with fluorescently labeled ddNTP complementary to the template DNA. The DNA fragments were then separated by capillary electrophores
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BIOL1133 Biological Sciences Laboratory Course
Practical 1
DNA sequencing and analysis
Date of practical: 24-1-2013
Plasmid DNA no.: 16
Abstract
Automated sequencing with dye-terminator was used in this practical. Different length of DNA fragments was synthesized with fluorescently labeled ddNTP complementary to the template DNA. The DNA fragments were then separated by capillary electrophoresis and computer found out the sequence. The sequence was then matched with GenBank using BLAST. Finally the sample DNA sequence was found to be Mus musculus zinc finger protein (Sna) mRNA, complete cds.
References
1. Saenger, Wolfram (1984). Principles of Nucleic Acid Structure. New York: Springer-Verlag
2. George KS, Zhao X, Gallahan D, et al. 1997. Capillary electrophoresis methodology for identification of cancer related gene expression patterns of fluorescent differential display polymerase chain reaction
3. Swerdlow H, Gesteland R. 1990. Capillary gel electrophoresis for rapid, high resolution DNA sequencing. Nucleic Acids Res 18: 1415–9.
4. Shendure JA, Porreca GJ, Church GM. 2008. Overview of DNA sequencing strat- egies. Curr Protoc Mol Biol Chapter 7: Unit 7.1.
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