Purdue University

• CHM 33901

• CHM33901 Primer Design Worksheet Name: Gabriella Keser Section and Group #: 011 group #1 My answers are written in blue Codon Chart Design primers for Abl-H396P (histidine to proline mutation) Your TA will do the first mutant on the board as an example and you will determine the primers for T315I mutant with your group. The nucleotide sequence of the Abl (229-511) construct that we are working with is identical to the kinase domain of the Bcr-Abl protein. The DNA sequence for the kinase domain is shown below. Genbank accession number for the full human Bcr-Abl protein: NM_005157 (https://www.ncbi.nlm.nih.gov/nuccore/NM_005157) Note: amino acid 229 corresponds to nucleotides 688-690 (not 685-687 as might be expected) because there are 3 bases prior to the start of the open reading frame (ORF). Before working with any nucleotide sequence, you should confirm that the DNA is in-frame and encodes the expected peptide or protein sequence using a DNA to protein translation tool (