• University of California, Davis

• PLB 112

 1. What are the floral phenotypes of the following plants: a. ap2 ap3 double mutant i. No A function, so C function expands to all four whorls. No B function. So carpels in all four whorls. b. 35S:AG transgenic plant i. C function ectopically expressed in all four whorls, will suppress A function in whorls 1 and 2. B function in whorls 2 and 3. So carpels, stamens, stamens, carpels. c. 35S:AP3 transgenic plant i. Expressing AP3 alone is not sufficient for ectopic B function. So the flower will look just like WT. d. 35S:AP3 35S:PI transgenic plant i. AP3 and PI together will provide ectopic B function in all four whorls. So petals in whorls 1 and 2, stamens in whorls 3 and 4. e. 35S:AP3 35:PI in a ag mutant transgenic plant i. AP3 and PI together will provide ectopic B function in all four whorls. No C function, so A function expands to all four whorls. So petals in all four whorls, repeating because of indeterminate phenotype of ag mutant 2. In which whorls of the flower will the following genes be expressed? a. AP3 gene in a superman mutant i. Whorls 2,3,4 b. AG mRNA in a sup mutant i. Whorls 3,4 c. microRNA172 precursor gene that targets the AP2 mRNA in a wild-type flower i. Whorls 3,4 d. AP2 gene in a wild-type flower i. Whorls 1,2,3,4 e. AP2 protein in a wild-type flower i. Whorls 1,2 3. Propose a set of genes that when ectopically expressed in the leaf will convert leaves to: a. Stamens i. B + C + SEP - therefore, AP3 + PI + AG + any SEP gene b. Sepals i. A + SEP. Therefore, AP1 + any SEP gene (AP2 is already expressed in leaves) 4. What is the evidence that the AG gene functions in the termination of the floral meristem? Describe the interactions between the LFY, WUS and AG genes that ensures that the floral meristem will terminate after making the carpels. a. In an ag mutant, the floral meristem persists, and this results in repeating floral organs. Also, AG is expressed in whorl 4, the region of the floral meristem that terminates in the carp