University of the Fraser Valley
BIOL
BIOL 3405
Question
Answered step-by-step
Q2. A 49 bp EcoRI fragment containing the somatostatin gene was...
Q2. A 49 bp EcoRI fragment containing the somatostatin gene was inserted into the vector
pWR590 shown below. The sequence of the inserted fragment is 5'-
AATTCGATCCTATCAACACGAAGTGAAAGTCTTACAACCCATGAATTCG - 3' 3'-
GCTAGGATAGTTGTGCTTCAC
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University of the Fraser Valley
BIOL
BIOL 3405
Question
Answered step-by-step
Q2. A 49 bp EcoRI fragment containing the somatostatin gene was...
Q2. A 49 bp EcoRI fragment containing the somatostatin gene was inserted into the vector
pWR590 shown below. The sequence of the inserted fragment is 5'-
AATTCGATCCTATCAACACGAAGTGAAAGTCTTACAACCCATGAATTCG - 3' 3'-
GCTAGGATAGTTGTGCTTCACTTTCAGAATGTTGGGTACTTAAGCTTAA - 5' Distances
between adjacent restriction sites in the pWR590 vector are indicated in the diagram. What are
the patterns of restriction digests with EcoRI (G^AATTC) or with MboI (^GATC) before and
after cloning the somatostatin gene into the vector? (E = EcoRI, M = MboI). (8 Marks).
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