Indipendent Learning Centre
BIOLOGY SBI4U Quiz 4.5.
Quiz Submissions - 4.5 - Assessment for Grading Quiz (12% of final grade
0 / 2 points
Write the DNA strand that is complementary to the strand shown below. Be sure to mark the
ends of your strand appropriately.
5’ -TACGGGCCCTTAGCTTTA-3’
Answer:
3' -
ATGCCCGGGAATCGAAAT
-5'
(3-A
...[Show More]
Indipendent Learning Centre
BIOLOGY SBI4U Quiz 4.5.
Quiz Submissions - 4.5 - Assessment for Grading Quiz (12% of final grade
Write the DNA strand that is complementary to the strand shown below. Be sure to mark the
ends of your strand appropriately.
5’ -TACGGGCCCTTAGCTTTA-3’
| Answer: |
3' -
ATGCCCGGGAATCGAAAT
-5' |
(3-ATGCCCGGGAATCGAAAT-5, 3'-
ATGCCCGGGAATCGAAAT-5', 3 -
ATGCCCGGGAATCGAAAT - 5, 3' -
ATGCCCGGGAATCGAAAT - 5', 3 -
ATGCCCGGGAATCGAAAT- 5, 3' -
ATGCCCGGGAATCGAAAT- 5', 3-
ATGCCCGGGAATCGAAAT -5, 3'-
ATGCCCGGGAATCGAAAT -5') |
| Question 2 |
4 / 4 points |
| Match the following terms having to do with DNA Replication. |
| __1__ |
helicase |
| __2__ |
gyrasen |
| __3__ |
DNA polymerase I |
| __4__ |
DNA ligase |
|
| 1. |
unwinds and unzip |
| 2. |
stabilizes the DNA
This prevents supe |
| 3. |
removes RNA prim |
| 4. |
rejoins the Okazak |
|
| Question 3 |
If the fragments of DNA shown below were to replicate with the origin of replication at the right
and the replication fork proceeding to the left, on which strand (A or B) would Okazaki
fragments be formed?
| Strand A: 5’ -ATCGATCCCTAG-3’ |
| Strand B: 3’ -TAGCTAGGGATC-5’ |
| Question 4 |
2 / 4.5 points |
| Match the following steps of protein synthesis. |
| __8__ |
Eventually, the ribosome reaches the stop codon and translation stops. |
| __5__ |
Translation begins when the small subunit of a ribosome recognizes the start codon. The ribosom |
| __2__ |
(1) |
Transcription begins as a short segment of the DNA double helix is unwound by RNA polymeras |
| __4__ |
When the transcript is completed, the mRNA undergoes post-transcriptional modification before
and the remaining exons are joined together, and a 5 cap and 3 poly-A tail are added. |
| __3__ |
(2) |
The RNA polymerase binds to the correct starting sequence of the gene at the promoter sequence |
| __1__ |
(3) |
An mRNA transcript is formed by the addition of RNA nucleotides complementary to the templa |
| __6__ |
Charged tRNA complexes attach to the ribosome. The anticodons of the tRNA match the codon o |
| __7__ |
(9) |
The amino acid chain (polypeptide) is released and folds into its final shape, forming the protein |
| __9__ |
(7) |
The amino acids are joined together as the ribosome moves along the mRNA. |
| Question 5 |
Match the following components with the biotechnology technique they play a role in.
| __2__ |
restriction endonuclease |
| __8__ |
dideoxynucleotides (ddNTP) |
| __3__ |
Taq polymerase |
| __1__ |
sodium dodecyl sulphate |
| __5__ |
ethidium bromide |
| __6__ |
polymerase chain reaction |
| __4__ |
agarose gel electrophoresis |
| __7__ |
plasmids |
|
| 1. |
extracting |
| 2. |
digesting/cutting |
| 3. |
elongating |
| 4. |
separating |
| 5. |
visualizing |
| 6. |
amplifying |
| 7. |
recombining |
| 8. |
sequencing/readin |
|
| Question 6 |
6.5 / 8 points |
___Transcription___(6.25 %)
begins as a short segment of the
___DNA___(6.25 %)
double helix is unwound by
___DNA Helicase___ (RNA polymerase)
. The RNA polymerase binds to the correct sequence of the gene at the
___promoter___ (promoter sequence)
. An
___Nascent___ (mRNA)
transcript is formed by the addition of RNA nucleotides complementary to the template
strand of DNA being copied.
When the transcript is completed, the mRNA undergoes
___post transcriptional modifications___ (post-transcriptional modification)
before moving to the cytoplasm.
___Spliceosome___ (Spliceosomes)
remove the
___introns___(6.25 %)
and the remaining
___exons___(6.25 %)
are joined together, and a 5 cap and 3 poly-A tail are added.
___Translation___(6.25 %)
begins when the small subunit of a
___ribosome___(6.25 %)
recognizes the start
___codon___(6.25 %)
. The ribosome is made up of
___rRNA___(6.25 %)
and proteins. Charged
___tRNA___(6.25 %)
complexes attach to the ribosome. The anticodons of the tRNA match the codon
over which the ribosome is sitting. The
___amino acids___(6.25 %)
are joined together as the ribosome moves along the mRNA. Eventually, the
ribosome reaches the stop codon and translation stops. The amino acid chain
(polypeptide) is released and folds into its final shape, forming the
___protein___(6.25 %)
coded for in the DNA.
Using the ß-hemoglobin DNA sequence shown here and the
genetic code provided in the table match the following to explain
the changes that you would predict based on the mutations.
[Show Less]
Access Full Document
Instant download after payment
Card Payments
₿
Crypto Accepted
-preview.png)
