DATE: 10/04/20NAME: Denelle PierreID #: 816013855COURSE CODE: BIOC 2169DEMONSTRATOR: VijaiINVERTASE SESSION 4-5PARTIAL PURIFICATION AND CHARACTERIZATION OF YEAST INVERTASE AIM: · To determine the effect of enzyme concentration on initial velocity for yeast invertase.· To investigate the effect of incubation time on product formation of yeast invertase.·
...[Show More]
DATE: 10/04/20
NAME: Denelle Pierre
ID #: 816013855
COURSE CODE: BIOC 2169
DEMONSTRATOR: Vijai
INVERTASE SESSION 4-5
PARTIAL PURIFICATION AND CHARACTERIZATION OF YEAST INVERTASE
AIM:
· To determine the effect of enzyme concentration on initial velocity for yeast invertase.
· To investigate the effect of incubation time on product formation of yeast invertase.
· To evaluate the effect of substrate concentration on enzyme activity in the presence and absence of the inhibitor urea for yeast invertase.
· To categorise the inhibitor urea on the basis of kinetic data (Lineweaver-Burk plot).
· To determine the kinetic parameters of yeast invertase; Vmax and Km.
· To conduct a SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on the enzyme fractions obtained from each purification step.
· To observe the purity differences of each collected enzyme fraction, using SDS-PAGE.
[Show Less]