• University of California, Berkeley

• BIO ENG 10

1. (_____ out of 2 points) The following picture is from Google Maps showing a plane in flight over Hyde Park. It was taken with a CCD camera that takes four images - one each for the blue, green, and red color channels, followed by another for luminosity, with a delay between each. The four images are then combined into one. The plane is a Boeing 747, and is 75 meters from tip to tail. You’ve narrowed down the type of camera that took the images to two types. Type A, which has a delay of 0.35 s between the different channels, and Type B, which has a delay of 0.06 s between the different channels. Which camera, Type A or Type B, was used to take this picture, and why? (Include an informal reference to a journal, web page, etc. if necessary.) Type B – A Boeing 747, according to the Microsoft Encarta Encyclopedia from 2000, has a cruising speed of 885 km/hr, which is standardized as 245 m/s. At this speed, the plane would travel roughly 86 meters in 0.35 seconds and roughly 15 meters in 0.06 seconds. Given the distance between each of the images of the plane following the delay, it is clear that the delay cannot be as large as 0.35 seconds, and it has to be the much smaller 0.06 second delay of camera Type B. 2. (_____ out of 3 points) Download and install the ImageJ image analysis software. On bcourses you will find an uncompressed TIF image file of pellets of bacteria in wells of a 96-well plate (“RFP assay - BioE 10.tif”). These wells have been photographed while exposed to UV light, using an appropriate filter, so the brightness of each pellet in the TIF corresponds to the quantity of “red” (e.g., photons of certain wavelengths) being emitted from the pellet and captured by the camera. The bacteria in each separate well have been transformed with a